Cardiovascular effects of beta-endorphin after microinjection into the nucleus tractus solitarii of the anaesthetised rat

Abstract

The cardiovascular effects of beta-endorphin after administration directly into the nucleus tractus solitari (NTS) of urethane-anaesthetised rats were investigated. Unilateral injection resulted in a U-shaped dose-response relationship with a fall in mean arterial pressure and heart rate occurring at low doses (less than 10 ng). No change in respiratory frequency was observed at any of the doses examined. The hypotensive effects of beta-endorphin were anatomically specific and restricted to the NTS. The depressor response was prevented and bradycardia reduced by naloxone (1 mg/kg s.c. or 10 ng injected into the NTS) and also by beta-endorphin antiserum (1:50 dilution) but not by antiserum to [Met5]enkephalin (1:50 dilution) applied locally into the NTS. The beta-endorphin antiserum caused a rise in blood pressure when administered alone. Conversely microinjection of antiserum to [Met 5]enkephalin resulted in a brief depressor response. Doses of beta-endorphin larger than 10 ng induced a rise in blood pressure accompanied by variable effects on heart rate. Similarly unilateral administration of Des-tyr-endorphin (100 pg) resulted in a blood pressure increase and [D-Ala2,Met5]enkephalin produced a dose-related pressor response and tachycardia. The results indicate that at least two separate endorphin systems are involved in cardiovascular control at the level of NTS, one being depressor in nature (beta-endorphin-like) and the other pressor ([met5]enkephalin-like).