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. 1982 Apr;18(4):361-8.
doi: 10.1007/BF02796336.

Rat serum stimulates serotonin N-acetyltransferase activity in pineal monolayer cultures

J Parr, V D Rowe

Rat serum stimulates serotonin N-acetyltransferase activity in pineal monolayer cultures

J Parr et al. In Vitro. 1982 Apr.

Abstract

The effects of rat serum on serotonin N-acetyltransferase (NAT) activity and indole synthesis in monolayer cultures of neonatal rat pineal glands was examined. The addition of 5% rat serum to these cultures resulted in stimulation of NAT activity equal to that obtained with optimal concentrations of the adrenergic agonist norepinephrine (NE). Rat serum also increased the synthesis of both N-acetylserotonin and melatonin from tryptophan. Stimulation of NAT activity by rat serum was partially blocked by metoprolol and propranolol, but not by phentolamine or butoxamine. The serum factor responsible for the stimulation was stable to both freezing and boiling. No significant amounts of epinephrine, norepinephrine, or dopamine were detected in the serum.

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References

    1. Life Sci. 1973 Oct 16;13(8):1089-103 - PubMed
    1. J Cell Physiol. 1965 Dec;66(3):325-33 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Apr;76(4):2057-61 - PubMed
    1. J Pharmacol Exp Ther. 1973 Sep;186(3):516-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 May;60(1):300-4 - PubMed

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