Stability of the cloned 'joint region' of herpes simplex virus DNA

Abstract

To isolate stable recombinants containing the 'joint region', or L-S junction, of herpes simplex virus DNA, the EcoRI restriction enzyme cleavage fragments were cloned into both coliphage lambda and plasmid vectors. The authentic joint region was found in the plasmid but not in the lambda vector. The plasmid-joint region recombinant DNAs appeared stable on limited passage. Subcloning the small BamHI L-S junction fragment into plasmid pBR322 gave rise to both stable and unstable recombinant DNAs.