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. 1982 Aug;79(16):4888-91.
doi: 10.1073/pnas.79.16.4888.

Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride

P C Sternweis, A G Gilman

Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride

P C Sternweis et al. Proc Natl Acad Sci U S A. 1982 Aug.

Abstract

Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates commercial preparations of various nucleotides and disposable glass test tubes, has been identified as Al3+. In the presence of 10 mM Mg2+ and 5 mM F-, AlCl3 causes activation of G/F with an apparent activation constant of approximately 1-5 muM. The requirement for Al3+ is highly specific; of 28 other metals tested, only Be2+ promoted activation of G/F by F-.

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