Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Nov;152(2):780-5.
doi: 10.1128/jb.152.2.780-785.1982.

Chromosomal location of a Bacillus subtilis DNA fragment uniquely transcribed by sigma-28-containing RNA polymerase

Chromosomal location of a Bacillus subtilis DNA fragment uniquely transcribed by sigma-28-containing RNA polymerase

F A Ferrari et al. J Bacteriol. 1982 Nov.

Abstract

A fragment of the Bacillus subtilis chromosome containing a gene whose transcription is dependent on sigma-28-containing RNA polymerase has been genetically mapped by means of an integrable plasmid. This gene resides on the chromosome adjacent to the stage 0 sporulation locus spo0E between metC and citL. The gene was insertionally inactivated by cloning an internal EcoRI-HindIII fragment in the integrable plasmid pJH101 and by inserting the plasmid into the chromosome by transformation. Transformants bearing an inactivated gene were indifferent to this inactivation for both growth and sporulation.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433-6 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. J Bacteriol. 1961 May;81(5):741-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 May;78(5):2762-6 - PubMed
    1. J Bacteriol. 1981 Oct;148(1):341-51 - PubMed

Publication types