doi: 10.1093/nar/10.16.4951.
Sequence requirements for the transcription of the rabbit beta-globin gene in vivo: the -80 region
- PMID: 6290997
- PMCID: PMC320844
- DOI: 10.1093/nar/10.16.4951
Item in Clipboard
Sequence requirements for the transcription of the rabbit beta-globin gene in vivo: the -80 region
Nucleic Acids Res.
.
Abstract
We have performed a detailed analysis of DNA sequences in the -80 region of the rabbit beta-globin gene that are required for transcription. A variety of rabbit beta-globin gene templates deleted at various sites in the -80 region were linked to an SV40-plasmid recombinant and introduced into HeLa cells; under these conditions the rabbit beta-globin gene is expressed from its own promotor. The results show that the conserved GGCCAATCT sequence is required for efficient transcription in vivo and further identify another sequence in the region from about -81 to -96 which is also required for transcription in vivo.
Similar articles
-
DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo.Nature. 1982 Jan 14;295(5845):120-6. doi: 10.1038/295120a0. Nature. 1982. PMID: 6276753
-
DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells.Cell. 1984 Aug;38(1):265-73. doi: 10.1016/0092-8674(84)90548-8. Cell. 1984. PMID: 6088069
-
DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.Proc Natl Acad Sci U S A. 1981 Mar;78(3):1411-5. doi: 10.1073/pnas.78.3.1411. Proc Natl Acad Sci U S A. 1981. PMID: 6262796 Free PMC article.
-
Use of long sequence alignments to study the evolution and regulation of mammalian globin gene clusters.Mol Biol Evol. 1993 Jan;10(1):73-102. doi: 10.1093/oxfordjournals.molbev.a039991. Mol Biol Evol. 1993. PMID: 8383794 Review.
-
Globin pseudogenes.Cell. 1982 Apr;28(4):683-4. doi: 10.1016/0092-8674(82)90045-9. Cell. 1982. PMID: 6284367 Review. No abstract available.
Cited by
-
The structure of an unusual leghemoglobin gene from soybean.EMBO J. 1983;2(3):449-52. doi: 10.1002/j.1460-2075.1983.tb01443.x. EMBO J. 1983. PMID: 11894962 Free PMC article.
-
The nucleotide sequence of the murine I-E beta b immune response gene: evidence for gene conversion events in class II genes of the major histocompatibility complex.EMBO J. 1984 Jun;3(6):1221-5. doi: 10.1002/j.1460-2075.1984.tb01956.x. EMBO J. 1984. PMID: 6086309 Free PMC article.
-
The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter.Nucleic Acids Res. 1983 Apr 25;11(8):2447-64. doi: 10.1093/nar/11.8.2447. Nucleic Acids Res. 1983. PMID: 6304653 Free PMC article.
-
A directly repeated sequence in the beta-globin promoter regulates transcription in murine erythroleukemia cells.Mol Cell Biol. 1990 Mar;10(3):972-81. doi: 10.1128/mcb.10.3.972-981.1990. Mol Cell Biol. 1990. PMID: 2304472 Free PMC article.
-
Tissue restricted and stage specific transcription is maintained within 411 nucleotides flanking the 5' end of the chicken alpha-skeletal actin gene.Nucleic Acids Res. 1986 Feb 25;14(4):1683-701. doi: 10.1093/nar/14.4.1683. Nucleic Acids Res. 1986. PMID: 3005979 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
