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Comparative Study
. 1982 Aug 25;10(16):5003-13.
doi: 10.1093/nar/10.16.5003.

Characterization of a highly repetitive family of DNA sequences in the mouse

T G Fanning
Comparative Study

Characterization of a highly repetitive family of DNA sequences in the mouse

T G Fanning. Nucleic Acids Res. .

Abstract

A large proportion (0.5-1%) of total mouse DNA is cleaved by Bam HI into fragments whose size is about 500 base pairs. A cloned member of this repetitive family of DNA sequences (BAM5 family) was sequenced by the dideoxy chain termination procedure and shown to contain 507 base pairs. The sequence exhibited no unusual or remarkable features. Repetitive sequences complementary to the cloned BAM5 fragment were found in rat DNA, but not in feline or human DNA. Restriction mapping suggested that many BAM5 sequences were components of much larger repetitive DNAs which were scattered throughout the mouse genome. The BAM5 sequences within the larger repetitive DNAs did not appear to be arranged tandemly or as members of scrambled tandem repeats. RNA homologous to the cloned BAM5 sequence was detected in cultured mouse cells, but not in cultured rat cells.

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