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. 1982 Sep;79(18):5503-7.
doi: 10.1073/pnas.79.18.5503.

Detection and cloning of human DNA sequences related to the mouse mammary tumor virus genome

R Callahan, W Drohan, S Tronick, J Schlom

Detection and cloning of human DNA sequences related to the mouse mammary tumor virus genome

R Callahan et al. Proc Natl Acad Sci U S A. 1982 Sep.

Abstract

Sequences related to the mouse mammary tumor virus (MMTV) genome have been detected in fragments of restricted human cellular DNA. These results were obtained by using recombinant DNA containing the MMTV proviral genome and lowering the stringency of blot-hybridization conditions. The MMTV genome also reacts with unique families of fragments in restricted cellular DNA from other mammalian species but not with salmon sperm DNA. A clone that reacted with labeled MMTV proviral DNA was selected from a human DNA library in Charon 4A. Under stringent conditions, a 3.7-kilobase MMTV-related EcoRI fragment of this clone hybridized with many of the same EcoRI restriction fragments of human cellular DNA detectable with MMTV proviral DNA under low-stringency conditions. Specific fragments of the human clone were shown to contain sequences related to the molecularly cloned gag, pol, and env regions of the MMTV genome.

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References

    1. Nature. 1972 Jan 7;235(5332):32-6 - PubMed
    1. Eur J Biochem. 1973 Jul 2;36(1):32-8 - PubMed
    1. Nature. 1974 Jun 7;249(457):565-7 - PubMed
    1. Cancer. 1974 Oct;34(4 Suppl):suppl:1421-6 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed

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