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. 1982 Sep;43(3):1032-7.
doi: 10.1128/JVI.43.3.1032-1037.1982.

In vitro transcription of two human rotaviruses

J Flores, J Myslinski, A R Kalica, H B Greenberg, R G Wyatt, A Z Kapikian, R M Chanock

In vitro transcription of two human rotaviruses

J Flores et al. J Virol. 1982 Sep.

Abstract

The RNA polymerase activities of a cultivatable (Wa) and a noncultivatable (DS-1) strain of human rotavirus were studied. Under optimal conditions, transcription of all of their RNA segments occurred, as evidenced by the hybridization of labeled transcripts to genomic RNA. Cross-hybridization between the two viruses showed that none of their 11 genes were completely homologous. The transcription products could be translated in vitro, yielding proteins with an electrophoretic pattern resembling that obtained with proteins labeled in vivo during infection with the Wa virus.

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