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. 1982 Nov 11;10(21):6945-55.
doi: 10.1093/nar/10.21.6945.

Drosophila topoisomerase I: isolation, purification and characterization

K Javaherian, Y C Tse, J Vega

Drosophila topoisomerase I: isolation, purification and characterization

K Javaherian et al. Nucleic Acids Res. .

Abstract

We have purified and characterized topoisomerase I from Drosophila melanogaster. The molecular weight of the enzyme is 135,000; 100,000, 90,000, and 65,000 molecular weight products result from degradation of the enzyme. The enzyme relaxes both positive and negative supercoiled DNA. Mg++ is not absolutely required, but stimulates the enzymatic activity considerably.

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