Identification and expression of a cloned yeast heat shock gene

Abstract

We have isolated the yeast HSP90 gene which encodes the Mr = 90,000 heat shock-inducible protein of this organism. When this gene is introduced into yeast on a multicopy plasmid vector, a dramatic increase is observed in the level of synthesis of the Mr = 90,000 heat shock-inducible protein. This protein overproduction is due to expression of the plasmid-borne HSP90 gene, which is under the same heat shock regulation as its chromosomal counterpart. The presence of an increased dosage of the HSP90 gene has no effect on the synthesis of the other major heat shock-inducible proteins and does not alter the heat shock-associated phenotype of thermal tolerance.