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. 1982 Oct 15;208(1):231-4.
doi: 10.1042/bj2080231.

Underestimation of metabolic rates owing to reincorporation of 14CO2 in the perfused rat liver

J F Tomera, P G Goetz, W M Rand, H Brunengraber

Underestimation of metabolic rates owing to reincorporation of 14CO2 in the perfused rat liver

J F Tomera et al. Biochem J. .

Abstract

(14)CO(2) production by perfused rat livers was simulated by infusing NaH(14)CO(3) into the perfusate. Recovery of label as (14)CO(2) gas + perfusate bicarbonate was 45-85%. Rates of (14)CO(2) exchange in the liver are 3-70 times greater than net rates of CO(2) production. Therefore (14)CO(2) reincorporation can lead to significant underestimations of rates of oxidation of (14)C-labelled substrates in liver.

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