doi: 10.1042/bj1690437.
A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes
- PMID: 629767
- PMCID: PMC1184186
- DOI: 10.1042/bj1690437
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A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes
Biochem J.
.
Abstract
A simple model of zonal gel filtration is analysed numerically to establish semiquantitatively how the capacity of a protein-ligand complex to survive gel filtration intact depends on several experimental variables and on the kinetic characteristics of the complex. The design of gel-filtration experiments aimed at detecting and partially purifying binding proteins is discussed.
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