Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Feb 1;169(2):437-40.
doi: 10.1042/bj1690437.

A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes

I A Nimmo, A Bauermeister

A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes

I A Nimmo et al. Biochem J. .

Abstract

A simple model of zonal gel filtration is analysed numerically to establish semiquantitatively how the capacity of a protein-ligand complex to survive gel filtration intact depends on several experimental variables and on the kinetic characteristics of the complex. The design of gel-filtration experiments aimed at detecting and partially purifying binding proteins is discussed.

PubMed Disclaimer

References

    1. Biochem J. 1977 Mar 15;162(3):659-64 - PubMed
    1. Biophys Chem. 1976 Sep;5(3):351-7 - PubMed
    1. Biochem Soc Trans. 1975;3(5):626-30 - PubMed
    1. Chromatogr Rev. 1970 Jan;12(1):88-107 - PubMed
    1. J Chromatogr. 1970 May 6;48(3):393-9 - PubMed