The influence of the ionic conductance on the relation between electron transport and proton-motive force in intact cells of Rhodopseudomonas capsulata

Abstract

1. The dependence of membrane potential (delta psi) on the rate of respiration in darkened intact cell suspensions of Rhodopseudomonas capsulata was distinctly non-linear: severe inhibition of respiration with either rotenone or KCN led to only a small drop in delta psi. 2. In the presence of 0.3 microMs carbonylcyanide p-trifluoromethoxyphenylhydrazone [CF3OPhzC(CN)2] the dependence of delta psi on respiratory rate became linear. Consequently, and particularly at lower concentrations of CF3OPhzC(CN)2, there was a pronounced, synergistic depression of the respiratory delta psi with CF3OPhzC(CN)2 and either rotenone or KCN. 3. Antimycin A, at a concentration which strongly inhibited the photosynthetic electron transport chain, only slightly lowered the light-induced delta psi in anaerobic cell suspensions. Antimycin and CF3OPhzC(CN)2 synergistically lowered delta psi generated by illumination. 4. The light-induced delta psi in anaerobic cells was only about 1.5-times larger than the respiratory-induced delta psi in darkened cells. Nevertheless it required approximately 16-times more CF3OPhzC(CN)2 to collapse the photosynthetic delta psi than the respiratory delta psi. 5. These results are discussed with reference to the ionic current/delta psi relation described in [J.B. Jackson (1982) FEBS Lett. 139, 139-143]. The unifying feature is that the intrinsic conductance of the cell membrane is strongly dependent on delta psi but the conductance due to CF3OPhzC(CN)2 is independent of delta psi.