Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Mar:64 Pt 3:697-700.
doi: 10.1099/0022-1317-64-3-697.

Production of human monoclonal antibody to X31 influenza virus nucleoprotein

D H Crawford, R E Callard, M I Muggeridge, D M Mitchell, E D Zanders, P C Beverley

Production of human monoclonal antibody to X31 influenza virus nucleoprotein

D H Crawford et al. J Gen Virol. 1983 Mar.

Abstract

In vitro stimulation of human peripheral blood mononuclear cells with X31 influenza virus antigen has been used to enrich for specific anti-X31 antibody-producing cells. Following Epstein-Barr virus transformation of these stimulated cells, a cell line which produces human antibody to X31 virus was derived and subsequently cloned. The cloned cells secrete and IgGl kappa antibody which is directed against the nucleoprotein of A type influenza virus. Culture supernatants contain 10 to 20 micrograms/ml of specific antibody which is now used as a standard for the ELISA assay used in our laboratory to detect antibodies to influenza virus.

PubMed Disclaimer

MeSH terms

LinkOut - more resources