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. 1983 Jan;3(1):143-6.
doi: 10.1128/mcb.3.1.143-146.1983.

Expression of hepatitis B virus surface antigen gene in cultured cells by using recombinant plasmid vectors

A Siddiqui

Expression of hepatitis B virus surface antigen gene in cultured cells by using recombinant plasmid vectors

A Siddiqui. Mol Cell Biol. 1983 Jan.

Abstract

By using a new host-vector system, expression of the gene coding for hepatitis B surface antigen has been studied. A subgenomic fragment of cloned hepatitis B viral DNA was inserted into the plasmid vector pSV010. Transfection of COS cells with the recombinant plasmid vector containing hepatitis sequences leads to the synthesis of hepatitis B surface antigen, which is released in the culture medium in the form of 22-nm particles similar to those found in the sera of hepatitis carriers.

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References

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