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. 1983 Mar 11;11(5):1295-308.
doi: 10.1093/nar/11.5.1295.

High efficiency polyoma DNA transfection of chloroquine treated cells

H Luthman, G Magnusson
Free PMC article

High efficiency polyoma DNA transfection of chloroquine treated cells

H Luthman et al. Nucleic Acids Res. .
Free PMC article

Abstract

Chloroquine treatment of rodent cells during the first hours of polyoma DNA transfection increase the fraction of cells expressing viral functions. The effect has been observed after DNA absorption using both the DEAE-dextran and calcium phosphate coprecipitation methods. Exposure to chloroquine increased the proportion of transfected mouse cells to approximately 40%. From a culture of one million such cells, microgram quantities of newly synthesized viral DNA could be isolated. Similarly, the transformation frequency of rat cells following polyoma DNA transfection was approximately 6-fold increased by chloroquine treatment. The effect of the compound was even more pronounced in transfections with linear forms of polyoma DNA, suggesting that chloroquine inhibits degradation of DNA absorbed by the cells.

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References

    1. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2163-7 - PubMed
    1. Nature. 1980 Jan 31;283(5746):445-53 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327-31 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 May;71(5):2077-81 - PubMed
    1. J Biol Chem. 1965 Jul;240:3123-31 - PubMed

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