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. 1978 Mar 14;523(1):1-8.
doi: 10.1016/0005-2744(78)90002-5.

Supercooled water as medium for enzyme reactions at subzero temperatures

P Douzou, P Debey, F Franks

Supercooled water as medium for enzyme reactions at subzero temperatures

P Douzou et al. Biochim Biophys Acta. .

Abstract

A water-in-oil emulsion technique was employed to investigate enzyme-catalyzed reactions at sub-zero temperatures in the supercooled liquid state. The results obtained with a monooxygenase (bacterial cytochrome P-450) clearly indicate the potentialities as well as the technical problems of the procedure which might be successfully used to investigate enzyme systems sensitive to cosolvents.

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