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. 1983 Jan;80(2):353-7.
doi: 10.1073/pnas.80.2.353.

Tumor induction by direct injection of cloned v-src DNA into chickens

Y K Fung, L B Crittenden, A M Fadly, H J Kung

Tumor induction by direct injection of cloned v-src DNA into chickens

Y K Fung et al. Proc Natl Acad Sci U S A. 1983 Jan.

Abstract

The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.

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References

    1. Virology. 1973 Apr;52(2):456-67 - PubMed
    1. Nature. 1977 Sep 22;269(5626):346-8 - PubMed
    1. Cell. 1977 Dec;12(4):983-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 - PubMed
    1. J Virol. 1978 Oct;28(1):45-52 - PubMed

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