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. 1983 Jan;80(2):477-81.
doi: 10.1073/pnas.80.2.477.

Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase

D J Jolly, H Okayama, P Berg, A C Esty, D Filpula, P Bohlen, G G Johnson, J E Shively, T Hunkapillar, T Friedmann

Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase

D J Jolly et al. Proc Natl Acad Sci U S A. 1983 Jan.

Abstract

We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.

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References

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