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. 1983 May 13;220(4598):725-7.
doi: 10.1126/science.6301016.

Gene transfer by polyoma-like particles assembled in a cell-free system

S N Slilaty, H V Aposhian

Gene transfer by polyoma-like particles assembled in a cell-free system

S N Slilaty et al. Science. .

Abstract

Empty capsids of polyoma virus interact with DNA in a cell-free system to form polyoma-like particles (PLP). The DNA in these particles is protected from the action of pancreatic deoxyribonuclease. Transfer of genetic information by PLP has been accomplished by using a restriction fragment containing the transforming sequences of polyoma DNA as a model gene. Infection of rat F111 cells by PLP containing these sequences results in DNA-mediated cellular transformation. Gene transfer by PLP is 50 to 150 times more efficient than by the calcium phosphate precipitation method.

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