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. 1983 Feb 1;128(2):393-7.
doi: 10.1016/0003-2697(83)90391-3.

Direct hybridization of labeled DNA to DNA in agarose gels

M Purrello, I Balazs

Direct hybridization of labeled DNA to DNA in agarose gels

M Purrello et al. Anal Biochem. .

Abstract

A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive probe, and the hybridization pattern visualized by autoradiography. This method is sensitive enough to detect single or low copy number DNA sequences in the genome of higher eucaryotes.

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