Transformation of a rabbit progesterone receptor from an 8S form to 5.5S and 4S forms

Abstract

The 8S form of the rabbit uterine progesterone receptor transforms slowly at 0 degree C to a 4S form with an intermediate 5.5S form. The transformation is accelerated by either heat, increased ionic strength and dilution of cytosol. The transformation, which is reversibly inhibited by sodium molybdate, is unrelated to total cytosolic alkaline phosphatase activity. The transformation is accompanied by a positive change in receptor surface charge and a decrease in the rate of progesterone dissociation. The stability of the 8S progesterone-receptor complex is reduced and the sedimentation coefficient increased by acidic conditions; acid does not affect the 4S receptor as drastically.