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. 1983 Apr 25;11(8):2287-302.
doi: 10.1093/nar/11.8.2287.

Expression in Saccharomyces cerevisiae of human interferon-alpha directed by the TRP1 5' region

M J Dobson, M F Tuite, J Mellor, N A Roberts, R M King, D C Burke, A J Kingsman, S M Kingsman
Free PMC article

Expression in Saccharomyces cerevisiae of human interferon-alpha directed by the TRP1 5' region

M J Dobson et al. Nucleic Acids Res. .
Free PMC article

Abstract

The complete 5' flanking region of the yeast TRP1 gene encoding N-(5'-phosphoribosyl)- anthranilate isomerase, a nonabundant protein, has been cloned and the nucleotide sequence data has been extended from -102 to -440. The CT block--CAAG structure common to all efficiently expressed yeast genes is altered in the 5' region of TRP1 and a sequence postulated to be involved in general amino acid regulation is absent. There are two possible TATA boxes at -224 and -262. TRP1, in common with HIS3, HIS4 and TRP5 has a region of dyad symmetry upstream of the coding sequence which may play a role in initiation of transcription. The relative efficiency of gene expression directed by the complete 5' TRP1 region was assessed by comparison with that of PGK by inserting a cDNA for a human interferon-alpha downstream of their respective 5' regions. The respective interferon yields indicate that their in vivo expression capabilities are a function of their 5' regions.

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References

    1. J Gen Virol. 1975 Dec;29(3):297-304 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Nucleic Acids Res. 1980 Jan 11;8(1):127-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 - PubMed
    1. Nature. 1981 Mar 26;290(5804):310-5 - PubMed

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