Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jun;80(11):3260-4.
doi: 10.1073/pnas.80.11.3260.

Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I

M J Lane, J C Dabrowiak, J N Vournakis

Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I

M J Lane et al. Proc Natl Acad Sci U S A. 1983 Jun.

Abstract

A direct approach to determining the sequence specificities of equilibrium binding drugs by using the DNase protection technique is described. The method utilizes singly end-labeled restriction fragments and partial digestion of the drug fragment complex with DNase I. Microdensitometry of autoradiograms produced after electrophoretic separation of digestion products allows determination of sequences that are affected by drug binding. The feasibility of the technique for locating small ligands bound to DNA and its eventual use as a quantitative thermodynamic approach to studying ligand binding to heterogeneous DNA as a function of sequence is illustrated by using actinomycin D and Netropsin.

PubMed Disclaimer

References

    1. J Antibiot (Tokyo). 1969 Sep;22(9):446-8 - PubMed
    1. J Bacteriol. 1972 May;110(2):667-76 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1911-4 - PubMed
    1. J Mol Biol. 1972 Jul 14;68(1):21-34 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1975;15(0):285-318 - PubMed

Publication types

LinkOut - more resources