AvaII and BglI restriction maps of bacteriophage Mu

Abstract

The AvaII and BglI restriction maps of bacteriophage Mu were derived by restriction analysis of a series of plasmid clones containing segments of Mu DNA which, in combination, covered the entire Mu genome. The plasmids analyzed included pKN36, pKN54, pKN62, pKN50, pKN35, pKN27, pKN48, pKN82, and pKN56 from the collection of W. Schumann and E. G. Bade, and pCM02, a newly constructed plasmid containing the rightmost internal EcoRI-PstI fragment of Mu DNA. BglI cuts Mu DNA at 23 sites, producing 24 fragments which range in size from 0.05 kb up to the approximately 7-kb fragment derived from the right end. AvaII cuts Mu DNA at 17 sites (including 2 within the G segment), producing fragments which range in size from 0.17 to 8.9 kb. The derived maps were confirmed by results of hybridization of 32P-labeled, nick-translated plasmid DNA to AvaII- and BglI-digested Mu DNAs. Evidence for modification of one of the AvaII sites in E. coli was obtained.