doi: 10.1016/s0022-2836(83)80333-7.
Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA)
- PMID: 6306257
- DOI: 10.1016/s0022-2836(83)80333-7
Item in Clipboard
Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA)
J Mol Biol.
.
Abstract
The genes infB, for translational initiation factor IF2, and nusA for a protein involved in transcription termination are carried on a 4.8 X 10(3) base-pair DNA fragment. This fragment also carries promoters capable of expressing both genes. The order of these genes with respect to the surrounding genes is pnp, rpsO, infB, nusA, argG. Transcription of the two genes is anticlockwise on the standard Escherichia coli map, i.e. directed towards the genes rpsO and pnp. The presence of infB on multicopy plasmids enhances IF2 protein and messenger RNA levels only two to threefold compared to the normal haploid level.
Similar articles
-
In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins.EMBO J. 1985 Feb;4(2):527-32. doi: 10.1002/j.1460-2075.1985.tb03660.x. EMBO J. 1985. PMID: 2990900 Free PMC article.
-
Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli.J Bacteriol. 1993 May;175(10):2880-7. doi: 10.1128/jb.175.10.2880-2887.1993. J Bacteriol. 1993. PMID: 8491709 Free PMC article.
-
Organization of the Thermus thermophilus nusA/infB operon and overexpression of the infB gene in Escherichia coli.Biochimie. 1997 Apr;79(4):195-203. doi: 10.1016/s0300-9084(97)83506-7. Biochimie. 1997. PMID: 9242984
-
E. coli ribosomal protein operons: the case of the misplaced genes.Cell. 1985 Aug;42(1):7-8. doi: 10.1016/s0092-8674(85)80093-3. Cell. 1985. PMID: 2410142 Review. No abstract available.
-
Translation mechanism in prokaryotes: structure and expression of Escherichia coli initiation factor IF3 gene.Biochem Soc Symp. 1982;47:95-112. Biochem Soc Symp. 1982. PMID: 6765497 Review. No abstract available.
Cited by
-
In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins.EMBO J. 1985 Feb;4(2):527-32. doi: 10.1002/j.1460-2075.1985.tb03660.x. EMBO J. 1985. PMID: 2990900 Free PMC article.
-
Transcriptional and post-transcriptional events trigger de novo infB expression in cold stressed Escherichia coli.Nucleic Acids Res. 2019 May 21;47(9):4638-4651. doi: 10.1093/nar/gkz187. Nucleic Acids Res. 2019. PMID: 30916329 Free PMC article.
-
Translation initiation factor IF2 of the myxobacterium Stigmatella aurantiaca: presence of a single species with an unusual N-terminal sequence.J Bacteriol. 1997 Apr;179(7):2348-55. doi: 10.1128/jb.179.7.2348-2355.1997. J Bacteriol. 1997. PMID: 9079922 Free PMC article.
-
Two translational initiation sites in the infB gene are used to express initiation factor IF2 alpha and IF2 beta in Escherichia coli.EMBO J. 1985 Jan;4(1):223-9. doi: 10.1002/j.1460-2075.1985.tb02339.x. EMBO J. 1985. PMID: 3894004 Free PMC article.
-
Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12.J Bacteriol. 1993 Nov;175(22):7178-88. doi: 10.1128/jb.175.22.7178-7188.1993. J Bacteriol. 1993. PMID: 8226664 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
