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. 1983 Jul;47(1):125-36.
doi: 10.1128/JVI.47.1.125-136.1983.

Capped and conserved terminal structures in human rotavirus genome double-stranded RNA segments

M Imai, K Akatani, N Ikegami, Y Furuichi

Capped and conserved terminal structures in human rotavirus genome double-stranded RNA segments

M Imai et al. J Virol. 1983 Jul.

Abstract

Both 3'- and 5'-terminal structures of human rotavirus genome double-stranded RNA segments were determined. RNAs were labeled at the 3'-termini with [32P]pCp by incubation with RNA ligase and at the 5'-termini with [32P]phosphate by polynucleotide kinase or, in the case of 5' caps, with 3H by chemical modification with [3H]NaBH4. Examination of radiolabeled termini released by digestion with several base-specific RNases revealed that rotavirus RNA segments are base paired end-to-end and contain the same terminal structures: (formula; see text)

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