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. 1983 Jul 11;11(13):4379-89.
doi: 10.1093/nar/11.13.4379.

Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe

I Schwartz, R A Klotsky, D Elseviers, P J Gallagher, M Krauskopf, M A Siddiqui, J F Wong, B A Roe
Free PMC article

Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe

I Schwartz et al. Nucleic Acids Res. .
Free PMC article

Abstract

A recombinant plasmid (designated pID2) carrying the E. coli gene for tRNAPhe has been isolated from a plasmid bank constructed by the ligation of a total EcoRI digest of E. coli K12 DNA into the EcoRI site of pACYC184 DNA. The plasmid was selected by virtue of its ability to complement a temperature-sensitive lesion in the gene (PheS) for the alpha-subunit of phenylalanyl-tRNA synthetase. Crude tRNA isolated from such transformants exhibited elevated levels of phenylalanine acceptor activity. The tRNAPhe gene has been localized within the first 300 base pairs of a 3.6 kb SalI fragment of pID2. The sequence of the gene and its flanking regions is presented.

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References

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