Comparative Study
doi: 10.1093/nar/11.13.4409.
A new restriction endonuclease from Acetobacter pasteurianus
- PMID: 6306590
- PMCID: PMC326055
- DOI: 10.1093/nar/11.13.4409
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Comparative Study
A new restriction endonuclease from Acetobacter pasteurianus
Nucleic Acids Res.
.
Free PMC article
Abstract
A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formula; see text) and cuts at the sites indicated by the arrows.
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