doi: 10.1126/science.6306768.
High efficiency DNA-mediated transformation of primate cells
- PMID: 6306768
- DOI: 10.1126/science.6306768
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High efficiency DNA-mediated transformation of primate cells
Science.
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Abstract
Tissue culture cells from several mammalian species, including three primate lines, were transfected with recombinant vectors carrying Escherichia coli xanthine-guanine phosphoribosyltransferase or Tn5 aminoglycoside phosphotransferase dominant selectable markers. Human HeLa and SV40-transformed xeroderma pigmentosum cells exhibited stable transformation frequencies of at least 10(-3) (0.1 percent). CV-1, an African green monkey kidney cell line, could be stably transformed with the exceptionally high frequency of 6 X 10(-2) (6 percent).
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