doi: 10.1042/cs0540329.
A radioimmunoassay for 1,25-dihydroxycholecalciferol
- PMID: 630809
- DOI: 10.1042/cs0540329
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A radioimmunoassay for 1,25-dihydroxycholecalciferol
Clin Sci Mol Med.
1978 Mar.
Abstract
1. 1alpha,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1alpha,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1alpha-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1alpha,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1alpha,25-dihydroxycholecalciferol.
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