Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Mar;54(3):329-32.
doi: 10.1042/cs0540329.

A radioimmunoassay for 1,25-dihydroxycholecalciferol

T L Clemens, G N Hendy, R F Graham, E G Baggiolini, M R Uskokovic, J L O'Riordan

A radioimmunoassay for 1,25-dihydroxycholecalciferol

T L Clemens et al. Clin Sci Mol Med. 1978 Mar.

Abstract

1. 1alpha,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1alpha,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1alpha-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1alpha,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1alpha,25-dihydroxycholecalciferol.

PubMed Disclaimer

Substances

LinkOut - more resources