Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Aug;80(15):4709-13.
doi: 10.1073/pnas.80.15.4709.

A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT

A D Miller, D J Jolly, T Friedmann, I M Verma

A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT

A D Miller et al. Proc Natl Acad Sci U S A. 1983 Aug.

Abstract

A cDNA corresponding to the human gene for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) has been ligated into murine retroviral vectors such that it is under the transcriptional control of viral long terminal repeats. Transfection of HPRT- cells followed by superinfection with various helper viruses has led to the rescue of chimeric virus capable of transmitting the HPRT+ phenotype to HPRT- rodent or human cells. These genetically transformed cells contain authentic human HPRT at levels similar to normal HPRT+ cells.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3307-11 - PubMed
    1. Cell. 1979 Jan;16(1):1-9 - PubMed
    1. Cell. 1980 Nov;22(2 Pt 2):479-88 - PubMed
    1. Radiat Res. 1961 Feb;14:213-22 - PubMed
    1. Nature. 1979 Jan 11;277(5692):108-14 - PubMed

Publication types