doi: 10.1128/jb.155.3.1105-1109.1983.
Chromate resistance plasmid in Pseudomonas fluorescens
- PMID: 6309741
- PMCID: PMC217804
- DOI: 10.1128/jb.155.3.1105-1109.1983
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Chromate resistance plasmid in Pseudomonas fluorescens
J Bacteriol.
1983 Sep.
Abstract
Chromate resistance of Pseudomonas fluorescens LB300, isolated from chromium-contaminated sediment in the upper Hudson River, was found to be plasmid specified. Loss of the plasmid (pLHB1) by spontaneous segregation or mitomycin C curing resulted in a simultaneous loss of chromate resistance. Subsequent transformation of such strains with purified pLHB1 plasmid DNA resulted in a simultaneous re-acquisition of the chromate resistance phenotype and the plasmid. When pLHB1 was transferred by conjugation to Escherichia coli, the plasmid still conferred chromate resistance.
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