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. 1983 Sep;155(3):1105-9.
doi: 10.1128/jb.155.3.1105-1109.1983.

Chromate resistance plasmid in Pseudomonas fluorescens

L H Bopp, A M Chakrabarty, H L Ehrlich

Chromate resistance plasmid in Pseudomonas fluorescens

L H Bopp et al. J Bacteriol. 1983 Sep.

Abstract

Chromate resistance of Pseudomonas fluorescens LB300, isolated from chromium-contaminated sediment in the upper Hudson River, was found to be plasmid specified. Loss of the plasmid (pLHB1) by spontaneous segregation or mitomycin C curing resulted in a simultaneous loss of chromate resistance. Subsequent transformation of such strains with purified pLHB1 plasmid DNA resulted in a simultaneous re-acquisition of the chromate resistance phenotype and the plasmid. When pLHB1 was transferred by conjugation to Escherichia coli, the plasmid still conferred chromate resistance.

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