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. 1983:101:228-45.
doi: 10.1016/0076-6879(83)01017-4.

Genetic applications of yeast transformation with linear and gapped plasmids

T L Orr-Weaver, J W Szostak, R J Rothstein

Genetic applications of yeast transformation with linear and gapped plasmids

T L Orr-Weaver et al. Methods Enzymol. 1983.

Abstract

Techniques for high frequency yeast transformation have been described. A double-strand break introduced by restriction enzyme cleavage can be used to direct a plasmid to integrate into a particular chromosomal locus. Plasmids containing a double-strand gap can be used in a straightforward method for the isolation and mapping of chromosomal alleles. These techniques extend the genetic applications of yeast transformation.

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