Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1983 Aug 25;11(16):5347-60.
doi: 10.1093/nar/11.16.5347.

The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences

L P Woudt, A Pastink, A E Kempers-Veenstra, A E Jansen, W H Mager, R J Planta
Free PMC article
Comparative Study

The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences

L P Woudt et al. Nucleic Acids Res. .
Free PMC article

Abstract

Sequences coding for histone H3 and H4 of Neurospora crassa could be identified in genomic digests with the use of the corresponding genes from sea urchin and X. laevis as hybridization probes. A 2.6 kb HindIII-generated N. crassa DNA fragment, showing homology with the heterologous histone H3-gene probes was cloned in a charon 21A vector. Using DNA from this clone as a homologous hybridization probe a 6.9 kb SalI-generated DNA fragment was isolated which in addition to the histone H3-gene also contains the gene coding for histone H4. Several lines of evidence demonstrate the presence of only a single histone H3- as well as a single histone H4-gene in N. crassa. The two genes are physically linked on the genome. DNA sequencing of the N. crassa histone H3- and H4-genes confirmed their identity and, in addition, revealed the presence of one short intron (67 bp) within the coding sequence of the H3-gene and even two introns (68 and 69 bp) within the H4-gene. The amino acid sequences of the N. crassa histones H3 and H4, as deduced from the DNA sequences, and those of the corresponding yeast histones differ only at a few positions. Much larger sequence differences, however, are observed at the DNA level, reflecting a diverging codon usage in the two lower eukaryotes.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. J Mol Appl Genet. 1983;2(1):83-99 - PubMed
    1. FEBS Lett. 1976 Jun 1;65(2):225-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
    1. Science. 1977 Apr 8;196(4286):180-2 - PubMed

Publication types

Associated data