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. 1983 Aug 25;11(16):5661-9.
doi: 10.1093/nar/11.16.5661.

Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli

H Weber, C Weissmann
Free PMC article

Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli

H Weber et al. Nucleic Acids Res. .
Free PMC article

Abstract

We describe a method for the formation of hybrid genes by in vivo recombination between two genes with partial sequence homology. DNA structures consisting of plasmid vector sequences, flanked by the alpha 2 interferon gene on the one side and a portion of the alpha 1 interferon gene (homology about 80%) on the other, were transfected into E. coli SK1592. Appropriate resistance markers allowed the isolation of colonies containing circular plasmids which arose by in vivo recombination between the partly homologous interferon gene sequences. Eleven different recombinant genes were identified, six of which encoded new hybrid interferons not easily accessible by recombinant DNA techniques.

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