Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jul;23(1):75-84.
doi: 10.1016/0378-1119(83)90218-4.

High-level expression in Escherichia coli of the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc protein

J A Lautenberger, D Court, T S Papas

High-level expression in Escherichia coli of the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc protein

J A Lautenberger et al. Gene. 1983 Jul.

Abstract

A plasmid, pJL6, was constructed that contains a unique ClaI site twelve codons beyond the bacteriophage lambda cII gene initiation codon. This site allowed us to fuse the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc gene to the amino-terminal portion of the cII gene. Transcription of the hybrid gene is controlled from the phage lambda pL promoter. When this promoter is derepressed, Escherichia coli cells harboring the chimeric plasmid produce a level of cII-myc fusion protein greater than 5% of total cellular protein. Antibodies raised by this protein immunoprecipitate the MC29 gag-myc gene product, P110gag-myc.

PubMed Disclaimer

LinkOut - more resources