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. 1983 Sep 24;11(18):6185-97.
doi: 10.1093/nar/11.18.6185.

Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy

T Ohno, N Takamatsu, T Meshi, Y Okada
Free PMC article

Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy

T Ohno et al. Nucleic Acids Res. .
Free PMC article

Abstract

The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170. (1982] and the complete nucleotide sequence has been established. The covalently closed circular single-stranded HSV RNA consists of 297 nucleotides. The secondary structure predicted for HSV contains 67% of its residues base-paired. The native HSV can possess an extended rod-like structure characteristic of viroids previously established. The central region of the native HSV has a similar structure to the conserved region found in all viroids sequenced so far except for avocado sunblotch viroid. The sequence homologous to the 5'-end of U1a RNA is also found in the sequence of HSV but not in the central conserved region.

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References

    1. Mol Cell Biol. 1982 Feb;2(2):161-70 - PubMed
    1. Science. 1981 Aug 7;213(4508):670-2 - PubMed
    1. Nucleic Acids Res. 1981 Dec 11;9(23):6527-37 - PubMed
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