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. 1983 Oct;80(20):6351-5.
doi: 10.1073/pnas.80.20.6351.

Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells

A Ochi, R G Hawley, T Hawley, M J Shulman, A Traunecker, G Köhler, N Hozumi

Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells

A Ochi et al. Proc Natl Acad Sci U S A. 1983 Oct.

Abstract

The rearranged immunoglobulin heavy (mu) and light (kappa) chain genes cloned from the Sp6 hybridoma cell line producing immunoglobulin M specific for the hapten 2,4, 6-trinitrophenyl were inserted into the transfer vector pSV2-neo and introduced into various plasmacytoma and hybridoma cell lines. The transfer of the mu and kappa genes resulted in the production of pentameric, hapten-specific, functional IgM.

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References

    1. Nature. 1983 Mar 24-30;302(5906):340-2 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Jan;80(2):417-21 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9 - PubMed
    1. Cell. 1983 Jul;33(3):717-28 - PubMed

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