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. 1983 Nov;156(2):952-5.
doi: 10.1128/jb.156.2.952-955.1983.

Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri

C Mouches, G Barroso, J M Bové

Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri

C Mouches et al. J Bacteriol. 1983 Nov.

Abstract

Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41.

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