Amino acid sequence of cytochrome c-553 from Desulfovibrio vulgaris Miyazaki

Abstract

The complete amino acid sequence of cytochrome c-553 from Desulfovibrio vulgaris Miyazaki has been determined. The protein has a single polypeptide chain containing 79 amino acid residues and has the typical characteristics of small mitochondrial cytochromes. Contrary to the expectation that the amino acid sequence of cytochrome c-553 from D. vulgaris Miyazaki is closely related to that from D. vulgaris Hildenborough, the two are not alike, except for the 20 NH2-terminal residues and the 4 carboxyl-terminal residues; however, the tryptic peptides obtained from the two cytochromes are similar to each other. The sequence of cytochrome c-553 from D. vulgaris Miyazaki resembles that of Pseudomonas cytochrome c-551. The phylogenetic situation of Desulfovibrio cytochrome c-553 in the phylogenetic tree of the cytochrome c super-family is discussed.