Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1983 Sep;10(2):119-29.
doi: 10.1016/0147-619x(83)90064-1.

Restriction endonuclease mapping of the root-inducing plasmid of Agrobacterium rhizogenes 1855

M Pomponi, L Spanò, M G Sabbadini, P Costantino
Comparative Study

Restriction endonuclease mapping of the root-inducing plasmid of Agrobacterium rhizogenes 1855

M Pomponi et al. Plasmid. 1983 Sep.

Abstract

The root-inducing plasmid of the agropine type Agrobacterium rhizogenes 1855 was mapped by means of the restriction endonuclease EcoRI. The circular arrangement of the more than 60 fragments generated by this enzyme was established by electrophoretic analysis of pBR322 clones harboring overlapping segments of pRi1855 derived by partial digestion with EcoRI. A large region of the plasmid comprising the T-DNA was mapped with two additional enzymes, BamHI and HindIII, by means of Southern blot hybridizations between the fragments generated by the three enzymes.

PubMed Disclaimer

Publication types

LinkOut - more resources