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. 1983 Oct 15;134(2):499-504.
doi: 10.1016/0003-2697(83)90330-5.

A simple spectrophotometric determination of formaldehyde and other aldehydes: application to periodate-oxidized glycol systems

G Avigad

A simple spectrophotometric determination of formaldehyde and other aldehydes: application to periodate-oxidized glycol systems

G Avigad. Anal Biochem. .

Abstract

A simple, rapid, and sensitive spectrophotometric assay procedure for the determination of as low as 2 microM solutions of formaldehyde and acetaldehyde using an alkaline 4-amino-5-hydrazino-3-mercapto-1,2,4-triazole reagent is described. The method is particularly useful for determination of these aldehydes (0.5-50 nmol) when produced by the periodate oxidation of various glycols and can be applied to the assay of dilute solutions of sugars or polyols.

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