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. 1983 Dec 16;65(1-2):183-90.
doi: 10.1016/0022-1759(83)90314-9.

An enzyme-linked immunoassay (ELISA) for measurement of lactoferrin

S V Hetherington, J K Spitznagel, P G Quie

An enzyme-linked immunoassay (ELISA) for measurement of lactoferrin

S V Hetherington et al. J Immunol Methods. .

Abstract

An enzyme-linked immunosorbent assay has been developed for quantitation of lactoferrin (LF) in body fluids. An indirect double-sandwich method was used which allows a sensitivity of 3 ng LF/ml in samples of polymorphonuclear cell lysates and serum. Mean LF content of serum was 0.307 +/- 0.066 micrograms/ml (n = 18). Mean LF content of polymorphonuclear cells was 4.90 +/- 1.48 micrograms/10(6) PMN. Concentrations of LF were similar in serum and in plasma of EDTA anticoagulated blood. Advantages of this method include its rapidity, and radioactivity is not required.

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