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. 1983 Oct;26(4):605-13.
doi: 10.1016/0090-6980(83)90197-1.

Studies on the conjugation of leukotriene B4 with proteins for development of a radioimmunoassay for leukotriene B4

R N Young, R Zamboni, J Rokach

Studies on the conjugation of leukotriene B4 with proteins for development of a radioimmunoassay for leukotriene B4

R N Young et al. Prostaglandins. 1983 Oct.

Abstract

Leukotriene B4 (LTB4) (I) has been converted to its N-(3-amino-propyl)amide derivative (III) and to its hydrazide derivative (VII) via LTB4 delta-lactone. The amide (III) was coupled with Bovine Serum Albumin using 1,5-difluoro-2,4-dinitrobenzene as coupling agent. The hydrazide (VII), was coupled with Hemocyanin (Keyhole Limpet) (KLH) using 6-N-maleimidohexanoic acid chloride as coupling agent.

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