High-performance liquid chromatographic determination of platinum (II) in plasma ultrafiltrate and urine: comparison with a flameless atomic absorption spectrometric method

Abstract

A technically simple, rapid and sensitive high performance liquid chromatographic assay for cis-dichlorodiammineplatinum (II) in human plasma ultrafiltrate and urine is described. The drug was chelated by exchange with diethyldithiocarbamate and extracted into chloroform. Nickel (II) was used as an internal standard which allows correction for the matrix effects observed with previous chromatographic and spectrometric methods. Chromatography was performed on a mu-Bondapak CN column and the eluent measured spectrophotometrically at 254 nm. Precision and reproducibility were both excellent and the detection limit was less than 50 ng/ml using only 1 ml of biological fluid.